Q1.
Non-green patches of the leaf obtained from the plant placed in sunlight do not turn blue-black. Does it indicate that there is no chlorophyll present in those patches?
Answer
Not necessarily. The correct conclusion is more careful: the non-green patches may not have sufficient chlorophyll to prepare enough starch for the iodine test to detect.
This is what Bhaskar recorded:
| Table 10.2: Presence of starch in green and non-green parts of the leaves of plants | |||
|---|---|---|---|
| S.No. | Light conditions for potted plant | Initial colours before iodine test | Final colours after iodine test |
| 1. | Plant kept in sunlight | Green and non-green patches on the leaf | Green patches of leaf turned blue-black (non-green patches unchanged) |
| 2. | Plant kept in the dark | Green and non-green patches on the leaf | No change in colour |
Why we must be careful: the iodine test detects starch, not chlorophyll. "No blue-black" only tells us that too little starch was made there to be detected. A small amount of chlorophyll may still be present in those patches — some coloured leaves even contain other pigments that hide the green.
Did you know? Some leaves look red, violet or brown because they contain more of these coloured pigments than green chlorophyll, which hides the green. Some of those pigments also help in photosynthesis — an iodine test on such a leaf can still turn blue-black.